TGM4: an immunogenic prostate-restricted antigen

TGM4: an immunogenic prostate-restricted antigen

Posted on by Faith
Prostate most cancers is the second main explanation for cancer-related demise in males within the USA; demise happens when sufferers progress to metastatic castration-resistant prostate most cancers (CRPC). Although immunotherapy with the Food and Drug Administration-approved vaccine sipuleucel-T, which targets prostatic acid phosphatase (PAP), extends survival for 2-Four months, the identification of latest immunogenic tumor-associated antigens (TAAs) continues to be an unmet want. 
We evaluated the differential expression profile of castration-resistant prostate epithelial cells that give rise to CRPC from mice following an androgen deprivation/repletion cycle. The expression ranges of a set of androgen-responsive genes had been additional evaluated in prostate, mind, colon, liver, lung, pores and skin, kidney, and salivary gland from murine and human databases.
The expression of a novel prostate-restricted TAA was then validated by immunostaining of mouse tissues and analyzed in major tumors throughout all human most cancers varieties in The Cancer Genome Atlas. Finally, the immunogenicity of this TAA was evaluated in vitro and in vivo utilizing autologous coculture assays with cells from wholesome donors in addition to by measuring antigen-specific antibodies in sera from sufferers with prostate most cancers (PCa) from a neoadjuvant scientific trial.
We recognized a set of androgen-responsive genes that might function potential TAAs for PCa. In specific, we discovered transglutaminase 4 (Tgm4) to be extremely expressed in prostate tumors that originate from luminal epithelial cells and solely expressed at low ranges in most extraprostatic tissues evaluated. Furthermore, elevated ranges of TGM4 expression in major PCa tumors correlated with unfavorable prognosis in sufferers. In vitro and in vivo assays confirmed the immunogenicity of TGM4.
We discovered that activated proinflammatory effector reminiscence CD8 and CD4 T cells had been expanded by monocyte-derived dendritic cell (moDCs) pulsed with TGM4 to a larger extent than moDCs pulsed with both PAP or prostate-specific antigen (PSA), and T cells primed with TGM4-pulsed moDCs produce practical cytokines following a major/enhance regiment or in vitro stimulation.
An IgG antibody response to TGM4 was detected in 30% of vaccinated sufferers, whereas fewer than 8% of vaccinated sufferers developed antibody responses to PSA or prostate-specific membrane antigen (PSMA).
TGM4: an immunogenic prostate-restricted antigen

Tissue engineered bovine saphenous vein extracellular matrix scaffolds produced through antigen elimination obtain excessive in vivo patency charges

Diseases of small diameter blood vessels embody the biggest portion of cardiovascular illnesses, with over 4.2 million folks present process autologous vascular grafting yearly. However, roughly one third of sufferers are ineligible for autologous vascular grafting resulting from lack of appropriate donor vasculature. Acellular extracellular matrix (ECM) scaffolds derived from xenogeneic vascular tissue have potential to function splendid biomaterials for manufacturing of off-the-shelf vascular graft able to eliminating the necessity for autologous vessel harvest.

A modified antigen elimination tissue (AR) course of, using aminosulfabetaine-16 (ASB-16) was used to create off-the-shelf small diameter (< three mm) vascular graft from bovine saphenous vein ECM scaffolds with considerably lowered antigenic content material, whereas retaining native vascular ECM protein construction and performance.

Elimination of native tissue antigen content material conferred graft-specific adaptive immune avoidance, whereas retention of native ECM protein macromolecular construction resulted in pro-regenerative mobile infiltration, ECM turnover and innate immune self-recognition in a rabbit subpannicular mannequin. Finally, retention of the fragile vascular basement membrane protein integrity conferred endothelial cell repopulation and 100% patency fee in a rabbit jugular interposition mannequin, comparable solely to Autograft implants.

Alternatively, the dearth of those necessary basement membrane proteins in in any other case similar scaffolds yielded a patency fee of solely 20%. We conclude that acellular antigen eliminated bovine saphenous vein ECM scaffolds have potential to function splendid off-the-shelf small diameter vascular scaffolds with excessive in vivo patency charges resulting from their low antigen content material, retained native tissue basement membrane integrity and preserved native ECM construction, composition and practical properties.

Nanoparticle Transfer Biosensors for the Non-Invasive Detection of SARS-CoV-2 Antigens Trapped in Surgical Face Masks

Detecting SARS-CoV-2 antigens in respiratory tract samples has turn out to be a widespread methodology for screening new SARS-CoV-2 infections. This requires a nasopharyngeal swab carried out by a skilled healthcare employee, which places pressure on saturated healthcare providers. In this manuscript we describe a brand new strategy for non-invasive COVID-19 analysis.

It consists of utilizing cellular biosensors for detecting viral antigens trapped in surgical face masks worn by sufferers. The biosensors are product of filter paper containing a nanoparticle reservoir. The nanoparticles switch from the biosensor to the masks on contact, the place they generate colorimetric indicators which might be quantified with a smartphone app.

Sample assortment requires sporting a surgical masks for 30 minutes, and the overall assay time is shorter than 10 min. When examined in a cohort of 27 sufferers with gentle or no signs, an space below the receiving working curve (AUROC) of 0.99 was obtained (96.2% sensitivity and 100% specificity). Serial measurements revealed a excessive sensitivity and specificity when masks had been worn as much as 6 days after analysis.

Surgical face masks are cheap and extensively accessible, which makes this strategy simple to implement wherever. Recent SARS-CoV-2 outbreak has been declared as a world well being emergency. It takes years to vaccinate the entire inhabitants to guard them from this lethal virus, therefore the administration of SARS-CoV-2 largely depends upon the widespread availability of an correct diagnostic check.

Towards addressing the unmet want of a dependable diagnostic check within the present work by using the ability of Systematic Evolution of Ligands by EXponential enrichment, a 44-mer G-quadruplex forming DNA aptamer towards spike trimer antigen of SARS-CoV-2 was recognized.

Rubella virus antibody
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Rubella virus E1 recombinant antigen
8806 100 ug
EUR 441.5
Description: This is Rubella virus E1 recombinant antigen for ELISA,WB.

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8807 100 ug
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80-1058 100 ug
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Rubella virus E2 recombinant antigen
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  • Category: Antigens, Rubella virus, Ag
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  • Category: Antigens, Rubella virus, Ag
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Rubella virus protein (Grade 1)
30-1330 1 ml
EUR 813
Description: Purified Native Rubella virus protein (Grade 1)

Rubella virus protein (Grade 2)
30-1331 1 mg
EUR 1682
Description: Purified Native Rubella virus protein (Grade 2)

Recombinant Rubella Virus E2 Protein
DAG514 1mg
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Rubella Virus E1 protein [GST]
DAGA-499 1mg
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Rubella virus RT PCR kit
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Recombinant Rubella Virus Rubella Capsid Protein, GST, E.coli-1mg
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Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-100ug
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Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-1mg
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Recombinant Rubella Virus Rubella E1 Protein, GST, E.coli-500ug
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Recombinant Rubella Virus Rubella E2 Protein, Untagged, E.coli-100ug
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Recombinant Rubella Virus Rubella E2 Protein, Untagged, E.coli-1mg
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Goat polyclonal antibody for Rubella virus
504 1 ml
EUR 311
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1701 1 ml
EUR 272.94
Description: This is goat polyclonal antibody against Rubella virus virions for WB, ELISA.

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1703 1 ml
EUR 289.25
Description: This is FITC conjugated goat polyclonal antibody against Rubella virus virions for WB, ELISA.

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1707 1 ml
EUR 289.25
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Rubella virus E1 mosaic recombinant antigen
00215-V-01mg 0,1 mg
EUR 267.5
  • Category: Antigens, Rubella virus, Ag
Description: Rubella virus E1 mosaic recombinant antigen a.a. 157-176/374-390/213-239.

Rubella virus E1 mosaic recombinant antigen
00215-V-1000ug 1000 ug
EUR 1282.5
  • Category: Antigens, Rubella virus, Ag
Description: Rubella virus E1 mosaic recombinant antigen a.a. 157-176/374-390/213-239.

Rubella virus capsid (C) recombinant antigen
00217-V-01mg 0,1 mg
EUR 267.5
  • Category: Antigens, Rubella virus, Ag
Description: Rubella virus capsid (C) recombinant antigen a.a. 1-123.

Rubella virus capsid (C) recombinant antigen
00217-V-1000ug 1000 ug
EUR 1282.5
  • Category: Antigens, Rubella virus, Ag
Description: Rubella virus capsid (C) recombinant antigen a.a. 1-123.

Mouse Anti Rubella Virus Monoclonal Antibody
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Recombinant Rubella Virus Capsid (33 kDa)
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Rubella virus One-Step PCR kit
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Rubella virus One-Step PCR kit
Oneq-H481-150R 150T
EUR 1060.6
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Rubella virus One-Step PCR kit
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EUR 751.75
  • Contact us in order to know the reactivity of the kit.
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Recombinant (E.Coli) Rubella Virus E2 protein
RP-1414 100 ug
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abx053898-96tests 96 tests
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abx364858-96tests 96 tests
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Mouse antibody for Rubella virus E1 glycoprotein
1712 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Rubella virus E1 glycoprotein for WB, ELISA.

Mouse antibody for Rubella virus E1 glycoprotein
1714 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Rubella virus E1 glycoprotein for WB, ELISA.

Mouse antibody for Rubella virus E1 glycoprotein
1715 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Rubella virus E1 glycoprotein for WB, ELISA.

Mouse antibody for Rubella virus E2 glycoprotein
1717 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Rubella virus E2 glycoprotein for WB, ELISA.

Goat Anti-Rubella Virus Virion Polyclonal Antibody
DPAB0218 1 ml
EUR 632

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DPAB1401 1 ml
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Recombinant Rubella Virus E1 Mosaic, His-tagged
DAG488 mg
EUR 1210

Recombinant Rubella Virus E1 mosaic (32 kDa)
DAG507 1mg
EUR 1438

Recombinant (E.Coli) Rubella Virus E1 Mosaic protein
RP-1413 100 ug
EUR 286

Recombinant (E.Coli) Rubella Virus Capsid C protein
RP-1415 100 ug
EUR 286

Bovine Rubella Virus Antibody IgG ELSA KIT
QY-E60087 96T
EUR 426

Human Rubella Virus (RV) IgG Rapid Test Kit
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Mouse Anti Rubella Virus Capsid Protein Monoclonal Antibody
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Mouse Anti Rubella Virus Glycoprotein E1 Monoclonal Antibody
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Mouse Anti Rubella Virus Glycoprotein E2 Monoclonal Antibody
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EUR 767

Goat Anti-Rubella virus (HPV77 strain) IgG, unlabeled
RUBL11-A 1 ml
EUR 324

Mouse Anti-Hepatitis A Virus (whole viral antigens) IgG
HAV13-M 100 ul
EUR 482

Hepatitis A Virus (HAV) antigens (FRhk4, inactivated) for ELISA
HAV15-N-100 100 ug
EUR 347

Goat Rubella virus antibody IgG ELSA KIT ELISA Kit
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EUR 521

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Bovine Rubella virus antibody IgA ELSA KIT ELISA Kit
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Bovine Rubella virus antibody IgM ELSA KIT ELISA Kit
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EUR 521

Anserine Rubella virus antibody IgG ELSA KIT ELISA Kit
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Anserine Rubella virus antibody IgA ELSA KIT ELISA Kit
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EUR 521

Anserine Rubella virus antibody IgM ELSA KIT ELISA Kit
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EUR 521

Canine Rubella virus antibody IgG ELSA KIT ELISA Kit
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EUR 521

Canine Rubella virus antibody IgA ELSA KIT ELISA Kit
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EUR 521

Canine Rubella virus antibody IgM ELSA KIT ELISA Kit
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EUR 521

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Porcine Rubella virus antibody IgA ELSA KIT ELISA Kit
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EUR 521

Porcine Rubella virus antibody IgM ELSA KIT ELISA Kit
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EUR 521

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Rabbit Rubella virus antibody IgA ELSA KIT ELISA Kit
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EUR 521

Rabbit Rubella virus antibody IgM ELSA KIT ELISA Kit
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EUR 521

Human anti-rubella virus(RV)antibody(IgG) ELISA Kit
CSB-E09529h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
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Human anti-rubella virus(RV)antibody(IgG) ELISA Kit
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  • EUR 500.00
  • EUR 3402.00
  • EUR 1820.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Qualitative indirect ELISA kit for measuring Human anti-rubella virus(RV)antibody(IgG) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Goat Anti-Rubella virus (HPV77 strain) IgG-Biotin conjugate
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EUR 347

Goat Anti-Rubella virus (HPV77 strain) IgG-FITC conjugate
RUBL11-FITC 1 ml
EUR 347

Goat Anti-Rubella virus (HPV77 strain) IgG-HRP conjugate
RUBL11-HRP 1 ml
EUR 347

Monoclonal Anti-Rubella virus (HPV72) E2 IgG, aff pure
RUBL12-M 100 ug
EUR 408

Monoclonal Anti-Rubella virus capsid protein IgG, aff pure
RUBL15-M 100 ug
EUR 408

Monoclonal Anti-Rubella virus core protein IgG, aff pure
RUBL16-M 100 ug
EUR 408

Mouse Rubella Virus Antibody IgG(RV-IgG )ELSA KIT
QY-E21685 96T
EUR 374

Varicella Zoster Virus (VZV/chickenpox) antigens/proteins (Ellen/HF cells)
VZV16-N-500 250 ul
EUR 408

ELISA kit for Human anti-rubella virus (RV)antibody (IgG)
EK0163 96 tests
EUR 603
Description: Enzyme-linked immunosorbent assay kit for quantification of Human anti-rubella virus (RV)antibody (IgG) in samples from serum, plasma, tissue homogenates and other biological fluids.

Guinea Pig Rubella virus antibody IgG ELSA KIT ELISA Kit
EGR0019 96Tests
EUR 521

Guinea Pig Rubella virus antibody IgA ELSA KIT ELISA Kit
EGR0020 96Tests
EUR 521

Guinea Pig Rubella virus antibody IgM ELSA KIT ELISA Kit
EGR0021 96Tests
EUR 521

Human Anti-Rubella Virus IgG ELISA kit, 96 tests,quantitative
510-100-HRG 1 kit
EUR 590

Human Anti-Rubella Virus IgM ELISA kit, 96 tests, quantitative
510-110-HRM 1 kit
EUR 590

Mouse Anti-Rubella Virus IgG ELISA kit, 96 tests, quantitative
510-120-MRG 1 Kit
EUR 773

Mouse Anti-Rubella Virus IgM ELISA kit, 96 tests, quantitative
510-130-MRM 1 Kit
EUR 773

Monoclonal Anti-Rubella virus envelop protein E1 IgG, aff pure
RUBL13-M 100 ug
EUR 408

Monoclonal Anti-Rubella virus envelop protein E2 IgG, aff pure
RUBL14-M 100 ug
EUR 408

Monoclonal Anti-Rubella virus structural glycoprotein E1 IgG, aff pure
RUBL17-M 100 ug
EUR 408

Mouse Monoclonal Anti-Hepatitis B Virus (HBsAg;AD & AY Antigens) IgG
AB-15010 100 ug
EUR 347

Rubella BR2S Antigen
30-1979 1 mg
EUR 1270

Rubella ELISA Kit
55R-RB025G 96 wells
EUR 301
Description: ELISA kit for detection of Rubella in the research laboratory

Rabbit Rubella IgG
QY-E30313 96T
EUR 374

Human anti-rubella virus IgM antibody(anti-RV IgM)ELISA Kit
GA-E1865HM-48T 48T
EUR 289

Human anti-rubella virus IgM antibody(anti-RV IgM)ELISA Kit
GA-E1865HM-96T 96T
EUR 466

Human anti-rubella virus IgG antibody(anti-RV IgG)ELISA Kit
GA-E1869HM-48T 48T
EUR 289

Human anti-rubella virus IgG antibody(anti-RV IgG)ELISA Kit
GA-E1869HM-96T 96T
EUR 466

Human anti-rubella virus IgM antibody,anti-RV IgM ELISA Kit
201-12-1849 96 tests
EUR 440
  • This anti-rubella virus IgM antibody ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human anti-rubella virus IgG antibody,anti-RV IgG ELISA Kit
201-12-1853 96 tests
EUR 440
  • This anti-rubella virus IgG antibody ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human anti-rubella virus IgM antibody, anti-RV IgM ELISA Kit
CSB-E09020h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Qualitative indirect ELISA kit for measuring Human anti-rubella virus IgM antibody, anti-RV IgM in samples from serum. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human anti-rubella virus IgM antibody, anti-RV IgM ELISA Kit
1-CSB-E09020h
  • EUR 500.00
  • EUR 3402.00
  • EUR 1820.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Qualitative indirect ELISA kit for measuring Human anti-rubella virus IgM antibody, anti-RV IgM in samples from serum. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Human anti-rubella virus IgM antibody(anti-RV IgM)ELISA Kit
QY-E02654 96T
EUR 361

Human anti-rubella virus IgG antibody(anti-RV IgG)ELISA Kit
QY-E02655 96T
EUR 361

Varicella Zoster Virus (VZV/chickenpox) antigens/proteins (VZ-10/MRC-5 cells)
VZV15-N-500 500 ul
EUR 408

ELISA kit for Human anti-rubella virus IgM antibody,anti-RV IgM
EK0162 96 tests
EUR 603
Description: Enzyme-linked immunosorbent assay kit for quantification of Human anti-rubella virus IgM antibody,anti-RV IgM in samples from serum, plasma, tissue homogenates and other biological fluids.

Canine Parainfluenza virus (CPiV) antigens (MDCK cells, inactivated, partially pure) for ELISA/Western
SEND45-N-100 100 ug
EUR 469

Monoclonal Varicella Zoster Virus (VZV/chickenpox) antigens IgG (pan, recognizes several VZV proteins)
VZV11-M 100 ug
EUR 482

Rubella IgG ELISA Kit
E4666-100
EUR 539

Rubella IgM ELISA Kit
E4667-100
EUR 539

Rubella 2-42 Protein
abx069921-1mg 1 mg
EUR 509
  • Shipped within 5-10 working days.

Rubella 2-6 Protein
abx069923-1mg 1 mg
EUR 509
  • Shipped within 5-10 working days.

Rubella IgM MAX Protein
abx160034-1mg 1 mg
EUR 3502
  • Shipped within 5-10 working days.

Human Cytomegalovirus, Toxoplasma, Rubella, Herpes simplex virus type II IgM Rapid Test Kit
abx092077-20tests 20 tests
EUR 342
  • Shipped within 5-12 working days.

The lead aptamer candidate (S14) was characterised totally for its binding, selectivity, affinity, construction and batch-to-batch variability by using various-biochemical, biophysical, and in silico methods. S14 has demonstrated a low nanomolar Okayd, confirming its tight binding to a spike antigen of SARS-CoV-2. S14 can detect as little as 2 nM of antigen.

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