DNA-protein and protein-protein interactions

January 3, 2021 0 Comments

Insights into gene regulation: From regulatory genomic components to DNA-protein and protein-protein interactions Transcription is orchestrated by non-coding regulatory components embedded in chromatin, which exist inside the bigger context of chromosome topology. Here, we evaluate latest insights into the capabilities of non-coding regulatory components and their protein interactors throughout transcription management. An image emerges through which …

Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests.

Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests.
May 10, 2020 0 Comments

To produce, purify, and characterize a polyclonal antibody against acrylamide (anti-AA) for an utility to immunochromatographic strip checks for AA. Polyclonal anti-AA was ready by injecting N-acryloxysuccinimideconjugated bovine serum albumin hapten-antigen into New Zealand white rabbits. The antibody was purified using protein A, characterised using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and conjugated with gold nanoparticles …