Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests.

Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests.

To produce, purify, and characterize a polyclonal antibody against acrylamide (anti-AA) for an utility to immunochromatographic strip checks for AA. Polyclonal anti-AA was ready by injecting N-acryloxysuccinimideconjugated bovine serum albumin hapten-antigen into New Zealand white rabbits. The antibody was purified using protein A, characterised using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and conjugated with gold nanoparticles … Read more Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests.