A narrative review of chimeric antigen receptor-T (CAR-T) cell therapy for lung cancer

Lung cancer represents one of the most typical and deadliest cancers on the planet. Chimeric antigen receptor-T cell (CAR-T) therapy which might acknowledge antigens in a significant histocompatibility advanced (MHC)-independent method offers a brand new method for tumor remedy. However, lung cancer, as a stable tumor, faces a number of formidable obstacles to adoptive cell switch, which incorporates inhibition of T-cell localization and suppression of T-cell operate.

Therefore, lung cancer fails to reply considerably to infusions of CAR-T cells in most trials till now. PubMed was researched utilizing the phrases “CAR-T” and “lung cancer” solely in English from 2000 by June 2020. We additionally included outcomes offered in worldwide conferences, such because the American Society of Clinical Oncology (ASCO) and the European Society for Medical Oncology (ESMO).

Besides, we discovered new progress in CAR-T therapy for stable tumors as a complement. To improve the efficacy and conquer the restrictions, we collected some functions in lung cancer. In latest years, there have been some enhancements in choosing the right goal and lowering toxicity. CAR-T know-how offers a wonderful manner for tumor remedy, which doesn’t depend upon MHC molecules and offers a brand new technique for the utilization of tumor targets.

Targeting totally different antigens and overcoming the stable barrier, there are some enhancements in responding considerably and lowering toxicity. CAR-T know-how will play a decisive position within the remedy of lung cancer. Determining Schistosoma mansoni an infection fee and depth is difficult because of the low sensitivity of the Kato-Katz (KK) take a look at that underestimates the true illness prevalence.

Circulating cathodic antigen (CCA) excreted in urine is continually produced by grownup worms and has been used as the idea of a easy, non-invasive level of care take a look at (POC-CCA) for Schistosoma mansoni infections. Although the abundance of CCA in urine is proportional to worm burden, the POC-CCA take a look at is marketed as a qualitative take a look at, making it tough to research the wide selection of an infection intensities.

This research was designed to match the prevalence and depth of S. mansoni by KK and POC-CCA and quantify, on recent and frozen (<-20°C) urine samples, CCA utilizing the visible scores and the ESEquant LR3 reader.

Considerations for Assessment and Deployment of Rapid Antigen Tests for Diagnosis of Coronavirus Disease 2019

Diagnostic testing is a crucial instrument to mitigate the extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, however molecular testing capability stays restricted. Rapid diagnostic checks (RDTs) that detect SARS-CoV-2 protein antigens (Ag) provide the potential to considerably increase testing capability and to permit frequent, large-scale, inhabitants screening. Testing is straightforward, fast (outcomes typically accessible inside 15 minutes), and relevant for analysis at level of care.

However, implementation of Ag RDTs requires an in depth understanding of take a look at efficiency and operational traits in every testing state of affairs and inhabitants being evaluated. Successful implementation of Ag RDTs on a big scale ought to mix testing with technical oversight and with scientific and public well being infrastructure, and would require manufacturing at ranges a lot greater than presently potential.

In this commentary, we offer detailed issues for Ag RDT evaluation and use circumstances to encourage and allow broader manufacturing and deployment.  The S. mansoni an infection charges inferred from POC-CCA and KK have been 40.7% and 9.4% respectively. Good correlations have been noticed between an infection intensities recorded by; i) the reader and visible scoring system on recent (Rho = 0.89) and frozen samples (Rho = 0.97), ii) the reader on recent urine samples and KK (epg) (Rho = 0.44).

Nevertheless, 238 POC-CCA optimistic kids have been unfavourable for KK, and sixteen of them had excessive ranges of CCA. The correlation between outcomes from the reader on recent and frozen samples was good (Rho = 0.85). On frozen samples, CCA was not detected in 55 samples that have been optimistic in recent urine samples.

A Biomimetic Aggregation-Induced Emission Photosensitizer with Antigen-Presenting and Hitchhiking Function for Lipid Droplet Targeted Photodynamic Immunotherapy

Photodynamic therapy (PDT) is a promising various method for efficient cancer remedy that’s related to an antitumor immune response. However, immunosuppression of the tumor microenvironment limits the immune response induced by PDT. Stimulation and proliferation of T cells is a crucial step for producing immune responses and is dependent upon the environment friendly presentation of tumor antigens and co-stimulatory molecules by antigen-presenting cells (APCs).

Here, biomimetic aggregation-induced emission (AIE) photosensitizers with antigen-presenting and hitchhiking skills (DC@AIEdots) are developed by coating dendritic cell (DC) membranes on the nanoaggregates of the AIEgens. Notably, the internal AIE molecules can selectively accumulate in lipid droplets of tumor cells, and the outer cell membrane can facilitate the hitchhiking of DC@AIEdots onto the endogenous T cells and improve the tumor supply effectivity by about 1.6 occasions.

Furthermore, DC@AIEdots can stimulate the in vivo proliferation and activation of T cells and set off the immune system. The potential functions of therapeutic brokers concentrating on lipid droplets for immunotherapy are indicated and a brand new hitchhiking method for drug supply is offered. Lastly, the research presents a photoactive and synthetic antigen-presenting platform for efficient T cell stimulation and cancer photodynamic immunotherapy.

Unusual aggregation property of recombinantly expressed cancer-testis antigens in mammalian cells

Transient expression of human intracellular proteins in human embryonic kidney (HEK) 293 cells is a dependable system for acquiring soluble proteins with biologically lively conformations. Contrary to standard ideas, we discovered that recombinantly expressed intracellular cancer-testis antigens (CTAs) confirmed frequent aggregation in HEK293 cells.

Although experimental subcellular localization of recombinant CTAs displayed correct cytosolic or nuclear localization, some proteins confirmed aggregated particles within the cell. This aggregative property was not noticed in recombinant housekeeping proteins.

BFP expression Adenovirus
AVP017	GenTarget	1x109 IFU/ml x 200ul	418.8 EUR
Description: pre-made BFP expression adenovirus, provided in DMEM medium.
Blunt E1 Expression Kit
abx098098-10rxns	Abbexa	10 rxns	560.4 EUR
Blunt E2 Expression Kit
abx098873-10rxns	Abbexa	10 rxns	560.4 EUR
RFP expression Adenovirus, in vivo ready
AVP001-PBS	GenTarget	1x1011 IFU/ml x 50ul	852 EUR
Description: pre-made RFP expression adenovirus, provided in PBS solution.
CFP expression Adenovirus, in vivo ready
AVP002-PBS	GenTarget	1x1011 IFU/ml x 50ul	852 EUR
Description: pre-made CFP expression adenovirus, provided in PBS solution.
GFP expression Adenovirus, in vivo ready
AVP011-PBS	GenTarget	1x1011 IFU/ml x 50ul	852 EUR
Description: pre-made GFP expression adenovirus, provided in PBS solution.
YFP expression Adenovirus, in vivo ready
AVP012-PBS	GenTarget	1x1011 IFU/ml x 50ul	852 EUR
Description: pre-made YFP expression adenovirus, provided in PBS solution.
BFP expression Adenovirus, in vivo ready
AVP017-PBS	GenTarget	1x1011 IFU/ml x 50ul	852 EUR
Description: pre-made BFP expression adenovirus, provided in PBS solution.
Rac1 Expression Vector Set
STA-454	Cell Biolabs	1 kit	1765.2 EUR
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.
RhoA Expression Vector Set
STA-456	Cell Biolabs	1 kit	1765.2 EUR
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.
Cdc42 Expression Vector Set
STA-455	Cell Biolabs	1 kit	1765.2 EUR
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.
h ADA Expression Lentivirus
LVP923	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker.
h IL6 Expression Lentivirus
LVP1144	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.
h CD4 Expression Lentivirus
LVP1152	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker.
h IL4 Expression Lentivirus
LVP1161	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker.
h IL7 Expression Lentivirus
LVP1162	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.
h KDR Expression Lentivirus
LVP1176	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker.
h FAS Expression Lentivirus
LVP1181	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker.
pAAV-MCS Expression Vector
VPK-410	Cell Biolabs	10 µg	776.4 EUR
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
h OCT4 expression Adenovirus, in vivo ready
AVP013-PBS	GenTarget	1x1011 IFU/ml x 50ul	852 EUR
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application.
h SOX2 expression Adenovirus, in vivo ready
AVP014-PBS	GenTarget	1x1011 IFU/ml x 50ul	852 EUR
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application.
h OCT4 expression Adenovirus
AVP013	GenTarget	1x109 IFU/ml x 200ul	418.8 EUR
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium.
h SOX2 expression Adenovirus
AVP014	GenTarget	1x109 IFU/ml x 50ul	418.8 EUR
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium.
h CDR1 Expression Lentivirus
LVP917	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker.
h CD47 Expression Lentivirus
LVP918	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker.
h ESR1 Expression Lentivirus
LVP924	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker.
r Syn1 Expression Lentivirus
LVP925	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker.
h NME4 Expression Lentivirus
LVP932	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker.
h EGFR Expression Lentivirus
LVP1142	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker.
h NRP1 Expression Lentivirus
LVP1145	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.
h MUC1 Expression Lentivirus
LVP1148	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.
h CD8a Expression Lentivirus
LVP1151	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker.
h PIGF Expression Lentivirus
LVP1154	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker.
h BRAF Expression Lentivirus
LVP1156	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker.
h ROS1 Expression Lentivirus
LVP1158	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker.
h CD3E Expression Lentivirus
LVP1163	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.
h KLK3 Expression Lentivirus
LVP1171	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker.
h NEK6 Expression Lentivirus
LVP1182	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker.
h NEK7 Expression Lentivirus
LVP1183	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker.
h LIN28 expression Adenovirus, in vivo ready
AVP015-PBS	GenTarget	1x1011 IFU/ml x 50ul	852 EUR
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application.
h LIN28 expression Adenovirus
AVP015	GenTarget	1x109 IFU/ml x 200ul	418.8 EUR
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in DMEM medium.
h GPNMB Expression Lentivirus
LVP921	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: GPNMB, containing a RFP-Blasticidin dual selection marker.
h DECR1 Expression Lentivirus
LVP927	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: DECR1, containing a RFP-Blasticidin dual selection marker.
h DECR2 Expression Lentivirus
LVP928	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: DECR2, containing a RFP-Blasticidin dual selection marker.
h NMRK1 Expression Lentivirus
LVP930	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: NMRK1, containing a RFP-Blasticidin dual selection marker.
h PITX3 Expression Lentivirus
LVP931	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: PITX3, containing a RFP-Blasticidin dual selection marker.
h HDAC1 Expression Lentivirus
LVP1143	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.
h VEGFB Expression Lentivirus
LVP1146	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.
h PARP1 Expression Lentivirus
LVP1149	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.
h IL3RA Expression Lentivirus
LVP1150	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker.
h PTCH1 Expression Lentivirus
LVP1155	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker.
h SART3 Expression Lentivirus
LVP1159	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: SART3, containing a RFP-Blasticidin dual selection marker.
h TIGIT Expression Lentivirus
LVP1160	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: TIGIT, containing a RFP-Blasticidin dual selection marker.
h EDNRA Expression Lentivirus
LVP1165	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: EDNRA containing a RFP-Blasticidin dual selection marker.
h Foxp3 Expression Lentivirus
LVP1172	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: Foxp3, containing a RFP-Blasticidin dual selection marker.
m RIKEN Expression Lentivirus
LVP1174	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for mouse target: RIKEN, containing a RFP-Blasticidin dual selection marker.
h APLP2 Expression Lentivirus
LVP1177	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: APLP2, containing a RFP-Blasticidin dual selection marker.
h PCSK9 Expression Lentivirus
LVP1178	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: PCSK9, containing a RFP-Blasticidin dual selection marker.
h NTSR1 Expression Lentivirus
LVP1179	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: NTSR1, containing a RFP-Blasticidin dual selection marker.
pscAAV-MCS Expression Vector
VPK-430	Cell Biolabs	10 µg	776.4 EUR
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
m CTAGA1 Expression Lentivirus
LVP916	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for mouse target: CTAGA1, containing a RFP-Blasticidin dual selection marker.
h SKIV2L Expression Lentivirus
LVP919	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a RFP-Blasticidin dual selection marker.
h SKIV2L Expression Lentivirus
LVP919-GP	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a GFP-Puromycin dual selection marker.
h TP5313 Expression Lentivirus
LVP926	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: TP5313, containing a RFP-Blasticidin dual selection marker.
h ELOVL5 Expression Lentivirus
LVP929	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: ELOVL5, containing a RFP-Blasticidin dual selection marker.
h MAGEA3 Expression Lentivirus
LVP1147	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.
h NANOS3 Expression Lentivirus
LVP1164	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: NANOS3, containing a RFP-Blasticidin dual selection marker.
h IL1RL1 Expression Lentivirus
LVP1168	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: IL1RL1, containing a RFP-Blasticidin dual selection marker.
h CTAG1B Expression Lentivirus
LVP1169	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: CTAG1B, containing a RFP-Blasticidin dual selection marker.
h MAGEA3 Expression Lentivirus
LVP1170	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: MAGEA3, containing a RFP-Blasticidin dual selection marker.
h COX7A1 Expression Lentivirus
LVP1173	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: COX7A1, containing a RFP-Blasticidin dual selection marker.
h BCL11B Expression Lentivirus
LVP1180	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: BCL11B, containing a RFP-Blasticidin dual selection marker.
pUC57-sgRNA expression vector
PVT18003	Lifescience Market	2 ug	309.6 EUR
H-Ras Expression Vector Set
STA-457	Cell Biolabs	1 kit	1765.2 EUR
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant.
ANPRA/Aequorin Expression vector
60641	BPS Bioscience	20 µg	900 EUR
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells.
h CEACAM5 Expression Lentivirus
LVP1153	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: CEACAM5, containing a RFP-Blasticidin dual selection marker.
h TNFSF11 Expression Lentivirus
LVP1157	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: TNFSF11, containing a RFP-Blasticidin dual selection marker.
BRAF, His-Tag (High Expression)
41237	BPS Bioscience	10 µg	375 EUR
Description: Human BRAF, also known as B-RAF1 and RAFB1, with 18 mutations to improve expression; S446A, S447A, I543A, I544S, I551K, Q562R, L588N, K630S, F667E, Y672S, A688R, L706S, Q709R, S713E, L716E, S720E, P722S, K723G. GenBank Accession #NM_004333, a.a. 444-723 with N-terminal His-tag, expressed in E. coli cells. MW = 33 kDa.
h TNFRSF1B Expression Lentivirus
LVP922	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: TNFRSF1B, containing a RFP-Blasticidin dual selection marker.
h KIAA1324 Expression Lentivirus
LVP1175	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: KIAA1324, containing a RFP-Blasticidin dual selection marker.
h KIAA0319L Expression Lentivirus
LVP920	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for human target: KIAA0319L, containing a RFP-Blasticidin dual selection marker.
GFP-Rac1 Expression Vector Set
STA-450	Cell Biolabs	1 kit	1939.2 EUR
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.
GFP-RhoA Expression Vector Set
STA-452	Cell Biolabs	1 kit	1939.2 EUR
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.
PhiC31 Integrase Expression Plasmid
FC200PA-1	SBI	10ug	496 EUR
GFP-Cdc42 Expression Vector Set
STA-451	Cell Biolabs	1 kit	1939.2 EUR
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.
Active Rac1 Expression Vector Set
STA-458	Cell Biolabs	1 kit	1765.2 EUR
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C.
pCMV-GFP-LC3 Expression Vector
CBA-401	Cell Biolabs	100 µL	1243.2 EUR
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.
h KRAS (G12D) Expression Lentivirus
LVP1166	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for a mutant (G12D) of human target: KRAS containing a RFP-Blasticidin dual selection marker.
Tet-on circRNA Expression Vector
PVT14643	Lifescience Market	2 ug	843.6 EUR
PinPoint Integrase Expression Plasmid
PIN200A-1	SBI	10ug	688 EUR
h TP53 (R175H) Expression Lentivirus
LVP1167	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for a mutant R175H) of human target: TP53, containing a RFP-Blasticidin dual selection marker.
pAAV-IRES-Neo Expression Vector
VPK-416	Cell Biolabs	10 µg	776.4 EUR
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-GFP Expression Vector
VPK-418	Cell Biolabs	10 µg	776.4 EUR
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-Bsd Expression Vector
VPK-419	Cell Biolabs	10 µg	776.4 EUR
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-Puro Expression Vector
VPK-415	Cell Biolabs	10 µg	776.4 EUR
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-Hygro Expression Vector
VPK-417	Cell Biolabs	10 µg	776.4 EUR
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
CEACAM5 CHO Cell Line (Low Expression)
78704-L	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.
Active H-Ras Expression Vector Set
STA-459	Cell Biolabs	1 kit	1765.2 EUR
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40.
CD36 HEK293 Cell Line (High Expression)
78526-H	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal HEK293 cell line stably expressing full length human CD36. The stable clonal cell line was selected for medium and high levels of CD36 expression compared to the parental HEK293 cell line.
CEACAM5 CHO Cell Line (High Expression)
78704-H	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.
AAV-1 Helper Free Expression System
VPK-410-SER1	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
AAV-2 Helper Free Expression System
VPK-410-SER2	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
AAV-3 Helper Free Expression System
VPK-410-SER3	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
AAV-4 Helper Free Expression System
VPK-410-SER4	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
AAV-5 Helper Free Expression System
VPK-410-SER5	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
AAV-6 Helper Free Expression System
VPK-410-SER6	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
Gryphon Retroviral Expression System I
ABP-RVK-10001	Allele Biotech	1 set	Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.
Gryphon Retroviral Expression System II
ABP-RVK-10002	Allele Biotech	1 set	Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.
B7-H3 CHO Cell Line (Low Expression)
78541-L	BPS Bioscience	2 vials	7150 EUR
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types.
AAV-DJ Helper Free Expression System
VPK-410-DJ	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.
B7-H3 CHO Cell Line (High Expression)
78541-H	BPS Bioscience	2 vials	7150 EUR
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types.
EP Reagent Biuret Reagent
1011601	Scientific Laboratory Supplies	1L	52.8 EUR
pRedPuro shRNA expression lentivector (EF1)
SI516A-1	SBI	10 ug	594 EUR
pAAV-MCS Promoterless Expression Vector
VPK-411	Cell Biolabs	10 µg	776.4 EUR
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pSMPUW-Neo Lentiviral Expression Vector
VPK-213	Cell Biolabs	10 µg	748.8 EUR
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
CD36 HEK293 Cell Line (Medium Expression)
78526-M	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal HEK293 cell line stably expressing full length human CD36. The stable clonal cell line was selected for medium and high levels of CD36 expression compared to the parental HEK293 cell line.
CEACAM5 CHO Cell Line (Medium Expression)
78704-M	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.
Human Myelin expression factor 2 (MYEF2)
1-CSB-EP015283HU	Cusabio	456.00 EUR 256.80 EUR 1570.80 EUR 672.00 EUR 1047.60 EUR 314.40 EUR	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Human Myelin expression factor 2(MYEF2),partial expressed in E.coli
scAAV-1 Helper Free Expression System
VPK-430-SER1	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
scAAV-2 Helper Free Expression System
VPK-430-SER2	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
scAAV-3 Helper Free Expression System
VPK-430-SER3	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
scAAV-4 Helper Free Expression System
VPK-430-SER4	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
scAAV-5 Helper Free Expression System
VPK-430-SER5	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
scAAV-6 Helper Free Expression System
VPK-430-SER6	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
pSMPUW-Puro Lentiviral Expression Vector
VPK-212	Cell Biolabs	10 µg	748.8 EUR
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
Protein Limb Expression 1 (LIX1) Antibody
20-abx318393	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Protein Limb Expression 1 (LIX1) Antibody
abx146234-100ug	Abbexa	100 ug	469.2 EUR
Protein Limb Expression 1 (LIX1) Antibody
abx025913-400ul	Abbexa	400 ul	627.6 EUR
Protein Limb Expression 1 (LIX1) Antibody
abx025913-80l	Abbexa	80 µl	343.2 EUR
scAAV-DJ Helper Free Expression System
VPK-430-DJ	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.
B7-H3 CHO Cell Line (Medium Expression)
78541-M	BPS Bioscience	2 vials	7150 EUR
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types.
Expression Negative Control Lentivirus (G418)
79902-G	BPS Bioscience	500 µl x 2	795 EUR
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_
pGreenPuro shRNA expression lentivector (CMV)
SI505A-1	SBI	10 ug	594 EUR
pGreenPuro shRNA expression lentivector (EF1)
SI506A-1	SBI	10 ug	594 EUR
pSMPUW-Hygro Lentiviral Expression Vector
VPK-214	Cell Biolabs	10 µg	748.8 EUR
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.
RAPAd® RSV Adenoviral Expression System
VPK-251	Cell Biolabs	1 kit	1243.2 EUR
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.
RAPAd® CMV Adenoviral Expression System
VPK-252	Cell Biolabs	1 kit	1243.2 EUR
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.
Claudin-6 CHO Cell Line (Low Expression)
78527-L	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.
EP Reagent Iodoplatinate Reagent
1046300	Scientific Laboratory Supplies	200ML	446.4 EUR
Myelin Expression Factor 2 (MYEF2) Antibody
abx235464-100ug	Abbexa	100 ug	577.2 EUR
Myelin Expression Factor 2 (MYEF2) Antibody
20-abx109333	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Myelin Expression Factor 2 (MYEF2) Antibody
20-abx113906	Abbexa	878.40 EUR 477.60 EUR	150 ul 50 ul
EP Reagent Molybdovanadic Reagent
1056700	Scientific Laboratory Supplies	100ML	52.8 EUR
Claudin-6 CHO Cell Line (High Expression)
78527-H	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.
AAV-DJ/8 Helper Free Expression System
VPK-410-DJ-8	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
CD7 CHO Cell Line (Medium or High Expression)-78324-H
78324-H	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant CD7 CHO-K1 cell lines expressing full-length human CD7 receptor (accession number: NM_006137.6). Surface expression of human CD7 was confirmed by flow cytometry. Each stable clonal cell line was selected for high or medium levels of CD7 expression to mimic different stages of cancer target cells with various CD7 expression levels.
CD7 CHO Cell Line (Medium or High Expression)-78324-M
78324-M	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant CD7 CHO-K1 cell lines expressing full-length human CD7 receptor (accession number: NM_006137.6). Surface expression of human CD7 was confirmed by flow cytometry. Each stable clonal cell line was selected for high or medium levels of CD7 expression to mimic different stages of cancer target cells with various CD7 expression levels.
Baculovirus expression vector pAc-g-CH3
30R-AB001	Fitzgerald	5 ug	739.2 EUR
Description: Baculovirus cassette vector pAc-l-CH3 for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant.
Genorise® Chicken CCL7 Expression Plasmid
GR148007	Genorise Scientific	10 µg	219 EUR
RAPAd® miRNA Adenoviral Expression System
VPK-253	Cell Biolabs	1 kit	1243.2 EUR
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.
CD95 CHO Cell Line (Medium or High Expression)-78499-H
78499-H	BPS Bioscience	2 vials	7150 EUR
Description: The CD95 CHO cell line is a recombinant clonal stable CHO cell line constitutively expressing full-length human CD95 receptor (accession number: NM_000043.3). Surface expression of human CD95 was confirmed by flow cytometry. Each clonal cell line was selected for different levels of CD95 expression (High or Medium) to mimic varying CD95 expression levels in cancer cells.
CD95 CHO Cell Line (Medium or High Expression)-78499-M
78499-M	BPS Bioscience	2 vials	7150 EUR
Description: The CD95 CHO cell line is a recombinant clonal stable CHO cell line constitutively expressing full-length human CD95 receptor (accession number: NM_000043.3). Surface expression of human CD95 was confirmed by flow cytometry. Each clonal cell line was selected for different levels of CD95 expression (High or Medium) to mimic varying CD95 expression levels in cancer cells.
pC001 - huLshC2C2- MBP for bacterial expression
PVT10645	Lifescience Market	2 ug	361.2 EUR
Exoenzyme C3 (Rho Inhibitor) Expression Vector
STA-460	Cell Biolabs	1 kit	860.4 EUR
Description: This mammalian expression vector codes for Exoenzyme C3, a Rho inhibitor.
Claudin-6 CHO Cell Line (Medium Expression)
78527-M	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal CHO cell line stably expressing full-length human claudin-6 (CLDN6 gene accession number NM_021195.5).  Surface expression of claudin 6 was confirmed by flow cytometry. The stable clonal cell line was selected for high, medium, or low levels of claudin-6 expression compared to the parental CHO-K1 cell line.
scAAV-DJ/8 Helper Free Expression System
VPK-430-DJ-8	Cell Biolabs	1 kit	1486.8 EUR
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
Platinum Retroviral Expression System, Ecotropic
VPK-300	Cell Biolabs	1 kit	1402.8 EUR
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-E Packaging cell line, which contains the gag and pol genes and an ecotropic envelope protein for viral packaging. Simply clone your gene of interest into the vector provided and transfect into the packaging cells.
Platinum Retroviral Expression System, Pantropic
VPK-302	Cell Biolabs	1 kit	1402.8 EUR
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-GP Packaging cell line which contains the gag and pol genes necessary for viral packaging. Simply clone your gene of interest into the vector provided and co-transfect into the packaging cells along with the included VSVG plasmid.
EP Reagent Methoxyphenylacetic Reagent
1053601	Scientific Laboratory Supplies	100ML	435.6 EUR
Cre Recombinase Expression Plasmid (pCMV-CRE)
CRE100A-1	SBI	10 µg	412 EUR
Flp recombinase expression HEK293 stable cell
SC006	GenTarget	2 x 106 cell/ml x 1ml	2670 EUR
Description: Flp recombinase stable cell line with Blasticidin resistance
Protein Limb Expression 1 (LIX1) Antibody (HRP)
20-abx308975	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Expression Negative Control Lentivirus (Puromycin)
79902-P	BPS Bioscience	500 µl x 2	795 EUR
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_
EP Reagent Phosphomolybdotungstic Reagent
1065000	Scientific Laboratory Supplies	100ML	201.6 EUR
BCMA CHO Recombinant Cell Line (Low Expression)
79500-L	BPS Bioscience	2 vials	6500 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human BCMA protein, also known as TNFRSF17, (Genbank #NM_001192). Surface expression of BCMA was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of BCMA expression, (H. M, L), to mimic different stages of cancer target cells with various BCMA expression levels.
CD19 CHO Recombinant Cell Line (Low Expression)
79561-L	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein, also known as B4 or CVID3, (Genbank #NM_001770). Surface expression of CD19 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD19 expression (High, Medium, Low) to mimic different stages of cancer target cells with various CD19 expression levels.
CD37/CHO Recombinant Cell Line (Low Expression)
79607-L	BPS Bioscience	2 vials	6500 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD37 protein, also known as GP52-40; TSPAN26; leukocyte antigen CD37 (Genbank #NM_001774). Surface expression of CD37 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD37 expression, (High, Medium, Low), to mimic different stages of cancer target cells with various CD37 expression levels.
CD38 CHO Recombinant Cell Line (Low Expression)
79615-L	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD20 protein, also known as MS4A1 (Genbank #NM_152866). Surface expression of CD20 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD20 expression (High, Medium) to mimic different stages of cancer target cells with various CD20 expression levels.
Protein Limb Expression 1 (LIX1) Antibody (FITC)
20-abx308976	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
Genorise® Feline EGF Expression Plasmid DNA
GR233001	Genorise Scientific	10 µg	199 EUR
Genorise® Chicken IL-6 Expression Plasmid
GR148001	Genorise Scientific	10 µg	219 EUR
Genorise® Chicken IL-4 Expression Plasmid
GR148003	Genorise Scientific	10 µg	219 EUR
Expression Negative Control Lentivirus (Hygromycin)
79902-H	BPS Bioscience	500 µl x 2	795 EUR
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_
Platinum Retroviral Expression System, Amphotropic
VPK-301	Cell Biolabs	1 kit	1402.8 EUR
Description: Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-A Packaging cell line, which contains the gag and pol genes and an amphotropic envelope protein for viral packaging. Simply clone your gene of interest into the vector provided and transfect into the packaging cells.
Myelin expression factor 2 Polyclonal Antibody
42263-100ul	SAB	100ul	399.6 EUR
pSMPUW-GFP-LC3 Lentiviral Expression Vector
LTV-801	Cell Biolabs	10 µg	1444.8 EUR
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.
RAPAd® Universal Adenoviral Expression System
VPK-250	Cell Biolabs	1 kit	1243.2 EUR
Description: Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification. The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed. The RAPAd® Universal Adenoviral Expression System (# VPK-250) contains a promoterless shuttle vector to allow you to insert the promoter of your choice along with your gene of interest to maximize gene expression.
EP Reagent Sulfomolybdic Reagent R3
1086500	Scientific Laboratory Supplies	1L	354 EUR
BCMA CHO Recombinant Cell Line (High Expression)
79500-H	BPS Bioscience	2 vials	6500 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human BCMA protein, also known as TNFRSF17, (Genbank #NM_001192). Surface expression of BCMA was confirmed by flow cytometry.
CD22 CHO Recombinant Cell Line (High Expression)
79557-H	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD22 protein (Genbank #NM_001771). Surface expression of CD22 was confirmed by flow cytometry. This clonal cell line was selected for medium level expression of CD22. A clone exhibiting higher levels of CD22 expression is also available (#79557-M). Please contact BPS Bioscience for additional information.
CD19 CHO Recombinant Cell Line (High Expression)
79561-H	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein, also known as B4 or CVID3, (Genbank #NM_001770). Surface expression of CD19 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD19 expression (High, Medium, Low) to mimic different stages of cancer target cells with various CD19 expression levels.
CD37/CHO Recombinant Cell Line (High Expression)
79607-H	BPS Bioscience	2 vials	6500 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD37 protein, also known as GP52-40; TSPAN26; leukocyte antigen CD37 (Genbank #NM_001774). Surface expression of CD37 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD37 expression, (High, Medium, Low), to mimic different stages of cancer target cells with various CD37 expression levels.
CD38 CHO Recombinant Cell Line (High Expression)
79615-H	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD20 protein, also known as MS4A1 (Genbank #NM_152866). Surface expression of CD20 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD20 expression (High, Medium) to mimic different stages of cancer target cells with various CD20 expression levels.
CD20 CHO Recombinant Cell Line (High Expression)
79624-H	BPS Bioscience	2 vials	7150 EUR
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD20 protein, also known as MS4A1 (Genbank #NM_152866). Surface expression of CD20 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD20 expression (High, Medium) to mimic different stages of cancer target cells with various CD20 expression levels.
Myelin Expression Factor 2 (MYEF2) Antibody (HRP)
20-abx108522	Abbexa	493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00 EUR	100 ug 1 mg 200 ug 20 ug 50 ug
PD-L1 / TCR Activator Mammalian Expression Kit
60610	BPS Bioscience	500 rxns.	590 EUR
Description: The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and human PD-L1 (GenBank Accession #NM_014143) in mammalian cells. The transfected cells can be used in conjunction with PD-1/NFAT Reporter/Jurkat T cells (BPS #60535) to study the interactions of PD-1 with PD-L1 ligand in a cellular context and screen for modulators of this signaling pathway._x000D_In this assay, PD-1/NFAT Reporter/Jurkat T cells are used as effector cells; HEK293 cells overexpressing PD-L1 (or PD-L2) and an engineered T cell receptor (TCR) activator are used as target cells. When these two cells are co-cultivated, TCR complexes on effector cells are activated by TCR activator on target cells, resulting in expression of the NFAT luciferase reporter. However, PD1 and PD-L1 (or PD-L2) ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-PD1 or anti-PD-L1 antibodies. PD1/PD-L1 neutralizing antibodies block PD1:PD-L1 interaction and promote T cell activation, resulting in reactivation of the NFAT responsive luciferase reporter.
PD-L2 / TCR Activator Mammalian Expression Kit
60620	BPS Bioscience	500 rxns.	565 EUR
Description: The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and human PD-L2 (GenBank Accession #NM_025239) in mammalian cells. The transfected cells can be used in conjunction with PD-1/NFAT Reporter/Jurkat T cells (BPS #60535) to study the interactions of PD-1 with PD-L2 ligand in a cellular context and screen for modulators of this signaling pathway._x000D_In this assay, PD-1/NFAT Reporter/Jurkat T cells are used as effector cells; HEK293 cells overexpressing PD-L1 (or PD-L2) and an engineered T cell receptor (TCR) activator are used as target cells. When these two cells are co-cultivated, TCR complexes on effector cells are activated by TCR activator on target cells, resulting in expression of the NFAT luciferase reporter. However, PD1 and PD-L1 or PD-L2 ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-PD1 or anti-PD-L1 antibodies. PD1, PD-L1, or PD-L2 neutralizing antibodies block PD1:PD-L1 and/or PD1:PD-L2 interaction and promote T cell activation, resulting in reactivation of the NFAT responsive luciferase reporter.
h INS-IGF2 (GFP-Puro) Expression Lentivirus
LVP699-GP	GenTarget	1x107 IFU/ml x 200ul	541.2 EUR
Description: Pre-made over-expression lentivirus for expressing human target: INS-IGF2 ( INS-IGF2 readthrough). It also contains a GFP-Puromycin dual selection marker.

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No important correlation was discovered between the aggregative and biophysical properties, reminiscent of hydrophobicity, contents of intrinsically disordered areas, and expression ranges, of CTAs.